Column Scale Up Factors

Column 1

Column 2

Internal Dia (mm)

Length (mm)

Flow Rate (ml/min)

Loading (mg)

Equivalent Column 2 Flow rate

Equivalent Column 2 Loading

Caution: Scale factors only apply to columns containing exactly the same phase. Scaling up to a larger particle size packing material will result in a loss of efficiency.

The flow rate scale factor is based on maintaining the same linear velocity through the column. In practice this will depend on the back-pressure encountered.